Release of clathrin from coated vesicles dependent upon a nucleoside triphosphate and a cytosol fraction
نویسندگان
چکیده
Calf-brain coated vesicles were incubated with ATP and a cytosol fraction. As much as 90% of the clathrin was selectively released within 10 min at 37 degrees C without detectable proteolysis. This uncoating process required the presence of both ATP and cytosol. Empty cages of clathrin could also be dissociated in a similar manner. A nonhydrolyzable analogue, 5'-adenylylimidodiphosphate (AMP-PNP), would not substitute for ATP. Clathrin was dissociated from coats in a form unable to reassemble into cages under standard conditions. These reactions may reflect a segment of a clathrin-coated vesicle cycle in which coats are removed from vesicles after budding.
منابع مشابه
RAPID COMMUNICATIONS Release of Clathrin from Coated Vesicles Dependent upon a Nucleoside Triphosphate and a Cytosol Fraction
Calf-brain coated vesicles were incubated with ATP and a cytosol fraction . As much as 90% of the clathrin was selectively released within 10 min at 37 °C without detectable proteolysis . This uncoating process required the presence of both ATP and cytosol . Empty cages of clathrin could also be dissociated in a similar manner . A nonhydrolyzable analogue, 5'-adenylylimidodiphosphate (AMPPNP), ...
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ورودعنوان ژورنال:
- The Journal of Cell Biology
دوره 93 شماره
صفحات -
تاریخ انتشار 1982